Overview
This protocol provides a method for analysis of cell surface antigens using direct immunofluorescence, i.e., when the antibody being used is directly labeled to a fluorescent dye. After staining, the cells can be analyzed immediately by flow cytometry or fixed and stored for later analysis. If appropriate instrumentation and antibodies are available, this method can be used for two-color analysis.
Details
Details of this protocol, Fluorescent Staining Of Cell Surface Antigens On Viable Cells Using Direct Immunofluorescence (Subscription Required), are located on a web site other than Biocompare Protocols.