Overview
This protocol describes a method for quantitative measurement of DNA in tissue culture cells using either propidium iodide (PI) or DAPI staining followed by flow cytometry. PI can be excited at 488 nm by the argon-ion laser, the most commonly used laser in flow cytometry. Alternatively, DAPI is best excited by a high-power UV laser, which is less commonly available.
Details
Details of this protocol, Propidium Iodide (PI) Or DAPI Staining Of Unfixed Tissue Culture Cells For Flow Cytometry (Subscription Required), are located on a web site other than Biocompare Protocols.