Delivery Of DsRNA Into Plants By VIGS Methodology - Subscription Required Protocol

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Title Delivery Of DsRNA Into Plants By VIGS Methodology - Subscription Required
Source CSH Protocols; 2006; doi:10.1101/pdb.prot4327

Overview

Virus-induced gene silencing (VIGS) uses a virus to deliver a sequence from a gene of interest into a host plant. The virus carrying the fragment of the gene of interest must be capable of replication if dsRNA is to be produced. One or two leaves are inoculated with Agrobacterium strains carrying the VIGS vector possessing the gene fragment. By Tessa M. Burch-Smith, Jennifer L. Miller and Savithramma P. Dinesh-Kumar.

Details

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