Overview
The method chosen for measuring the content of cyclic adenosine 3',5'- monophosphate (cyclic AMP or cAMP) in splenic B lymphocytes (B cells) is an enzyme-linked immunoassay system developed by Amersham Biosciences. An overview of the assay, cAMP Biotrak EIA, and its instructions, Biotrak Protocol, are available through the indicated links. There are two ligand additions to allow either simultaneous addition of two ligands (premixed) or a response period between additions of single ligands. The procedure described below provides a sufficient sample for several determinations of cAMP using Amersham’s acetylation protocol.
Details
Details of this protocol, Preparation Of B-Lymphocyte Lysates For Cyclic AMP Determination For Dual Ligand Screen, are located on a web site other than Biocompare Protocols.