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Assay Of Intracellular Free Calcium In RAW 264.7 Cells For Ligand Screen (with Fluoroskan Ascent)
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| Protocol Information |
| Title |
Assay Of Intracellular Free Calcium In RAW 264.7 Cells For Ligand Screen (with Fluoroskan Ascent)   |
| Source |
The Alliance for Cellular Signaling |
| Overview |
| This protocol describes a method to assess concentrations of free cytoplasmic
calcium (Ca2+) in cultured RAW 264.7 cells. This objective is accomplished with the
Ca2+-sensitive fluorescent dye, fluo-3, which permeates cells as an ester and is
hydrolyzed in the cell to its Ca2+-sensitive acidic form |
| Details |
Details of this protocol, Assay Of Intracellular Free Calcium In RAW 264.7 Cells For Ligand Screen (with Fluoroskan Ascent), are located on a web site other than Biocompare Protocols.
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