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Production of Mouse T Cell Hybridomas
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| Protocol Information |
| Title |
Production of Mouse T Cell Hybridomas  |
| Source |
Ada M. Kruisbeek, Netherlands Cancer Institute, Amsterdam, The Netherlands |
| Overview |
| T cell hybridomas can be obtained by fusing activated T cells with tumor cells. A heterogeneous population of hybridomas can be cloned by limiting dilution to obtain hybridomas that express specificity to one T cell receptor (TCR). This unit describes cell fusion and selection of T cell hybridomas. A protocol is provided for screening of T cell hybridomas for expression of the CD3-TCR complex by flow cytometry analysis. Those hybridomas expressing a CD3-TCR complex are then further tested for expression of antigen specificity by screening for specific antigen-induced lymphokine production. |
| Details |
Details of this protocol, Production of Mouse T Cell Hybridomas, are located on a web site other than Biocompare Protocols.
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