RT-PCR

RT-PCR related protocols

• 5’ Rapid Amplification Of CDNA Ends (RACE)

  Source:

• Amplification Of RNA: High-Temperature Reverse Transcription And DNA Amplification With A Magnesium-Activated Thermostable DNA Polymerase (Subscription Required)

  Source: CSH Protocols; 2006; doi:10.1101/pdb.prot4115

• Competitive RT-PCR: Estimation Of Copy Number (Subscription Required)

  Source: CSH Protocols; 2006; doi:10.1101/pdb.prot4111

• Competitive RT-PCR: Preparation Of Competitor RNA (Subscription Required)

  Source: CSH Protocols; 2006; doi:10.1101/pdb.prot4110

• Gene-Specific RT-PCR

  Source: National Cancer Institute, Molecular Profiling Initiative

• Protocol For Real-Time RT-PCR

  Source: Primer Bank Harvard University

• Real-Time RT-PCR: CDNA Synthesis (Subscription Required)

  Source: CSH Protocols; 2006; doi:10.1101/pdb.prot4114

• Relative RT-PCR: Determining The Linear Range Of Amplification And Optimizing The Primers:Competimers Ratio (Subscription Required)

  Source: CSH Protocols; 2006; doi:10.1101/pdb.prot4109

• Reverse Transcriptase In Situ PCR Technique (Subscription Required)

  Source: CSH Protocols; 2006; doi:10.1101/pdb.prot4119

• Semiquantitative RT-PCR For Phenotype Analysis Of RNAi-Treated Drosophila Embryos

  Source: CSH Protocols; 2006; doi:10.1101/pdb.prot4518

• RT-PCR Analysis

  Source: Mike A. Dyer, Cepko Lab, Harvard University

• Optimized Welsh DDRT-PCR Protocol

  Source: Mirta Grifman, The Hebrew University of Jerusalem