Xenopus Embryo Manipulation

Xenopus Embryo Manipulation related protocols

• Animal Cap Assay (AC Assay)

  Source: Hiroki Kuroda, De Robertis Lab, Howard Hughes Medical Institute, UCLA

• Cryosectioning Xenopus Embryos

  Source: Frank Conlon Lab, Dept of Genetics & Biology - University of North Carolina - Chapel hill

• De-Jellying Eggs

  Source: Harland Lab, Dept. Molecular & Cell Biology, University of California, Berkeley

• Dissociation-And-Reaggregation Of Animal Cap Cells

  Source: Kuroda et al. Genes Dev. 19, 1022-1027 (2005)

• Embryo Lysates & Immunoprecipitation:

  Source: Klymkowsky Lab, Molecular, Cellular & Developmental Biology, UC Boulder.

• Fixation Of Embryos

  Source: Harland Lab, Dept. Molecular & Cell Biology, University of California, Berkeley

• TUNEL Staining

  Source: Harland Lab, Dept. Molecular & Cell Biology, University of California, Berkeley

• Whole Embryo Protein Extraction With Freon

  Source: Frank Conlon Lab, Dept of Genetics & Biology - University of North Carolina - Chapel hill