ApoBrdU DNA Fragmentation Assay Kit from MBL International

Description

The TUNEL-based assay kit provides complete components including positive and negative control cells for convenient detection of DNA fragmentation in cultured cells and tissue sections. The incorporation of BrdU in the procedure provides greater sensitivity than biotinylated or digoxigenylated methods. Results can be analyzed by fluorescence microscopy or flow cytometry.

Internucleosomal DNA fragmentation is a hallmark of apoptosis in mammalian cells. MBL’s Apo-BrdU In Situ DNA Fragmentation Assay Kit provides complete components including positive and negative control cells for conveniently detecting DNA fragmentation by fluorescence microscopy or flow cytometry. The kit utilizes Br-dUTP (bromolated deoxyuridine triphosphate nucleotides) which is more readily incorporated into DNA strand breaks than other larger ligands (e.g., fluorescein, biotin or digoxigenin). The greater incorporation gives rise to brighter signal when the Br-dUTP sites are identified by a fluorescein labeled anti-BrdU monoclonal antibody.