LA TAQ W/GC BUFFER 125 U
from
Takara Bio USA
Description
Takara LA Taq™ is a mixture of Taq Polymerase with a proofreading polymerase optimized for amplification of long DNA templates. Using LA Taq™, routine extension to 20 kb is possible (and up to 48 kb on some templates), with less optimization than other long PCR systems. Because of the presence of the proofreading polymerase, fidelity is significantly better (6.5X) than Taq Polymerase alone. The GC-optimized Buffers I and II are specifically designed to amplify DNA templates with high GC content or a significant amount of secondary structure. GC Buffer I is recommended for amplification of fragments ≥5kb, whereas GC Buffer II is recommended for 2-3 kb fragments with up to 73% GC content.