BLOCK-iT™ Transfection Optimization Kit
from
Invitrogen
Description
The BLOCK-iT™ Transfection Optimization Kit is designed to optimize RNAi transfection using controls for transfection and viability. Each kit contains a BLOCK-iT™ Fluorescent Oligo, which provides:
- A strong, easily detectable FITC signal indicating transfection efficiency
- Chemical modifications for a clearer, more persistent cellular signal than labeled siRNA provides
- Proven correlation of transfection efficiency with Stealth™ RNAi and traditional siRNA molecules
This makes BLOCK-iT™ Fluorescent Oligo an ideal indicator of transfection efficiency for RNAi experiments. In addition, the kit contains a Positive Control Stealth™ RNAi molecule directed at human p53 and a Scrambled Stealth™ RNAi molecule as a negative control. To assay cell viability, Dead Cell Dye is included. This dye colors dead cells as red, thereby allowing easy identification of non-viable cells in a tissue culture plate. Using these reagents together allows you to both optimize transfection conditions and have a functional positive control in every RNAi experiment you perform.