Soil DNA isolation kits readily extract total DNA material from various soil samples, which can harbor a variety of soil-based organisms such as bacteria, fungi, and other microbes. Features of these kits include reagents tailored to remove environmental contaminants such as metals and polysaccharides that can interfere with downstream molecular applications. These ready-to-use kits for soil microbial DNA purification can be well suited for workflows in microbiome sequencing and metagenomics.