Rat Tissue Western Blot

Rat Tissue Western Blot

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Recent Product Review

  • NTF97 antibody for Western Blotting

    NTF97 antibody for Western Blotting

    The hedgehog signaling plays a critical role in embryonic development. It also controls adult tissue homeostasis and deregulation of this pathway results in basal cell carcinoma and medulloblastoma. A unique feature of this pathway is that it requires a tiny antenna like organelle called the primary cilium for signal transduction. My goal is to understand the molecular mechanism of hedgehog signal transduction at the primary cilium. This involves detecting protein-protein interactions of the pathway components, analysis of localization patterns during signal transduction, and transcriptional output measurements. In the present experiment, knockdown of hedgehog pathway components was performed and a marker was needed to judge loading of samples for Western Blotting. NTF97 blotting was done to check if this antibody works in 3T3 cells so that it can be used at later stages.
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    The aim of this study is to study the expression of enzymes regulating the retinoic acid pathway in a mouse model of colitis-associated colon cancer model. In this experiment, the expression of ALDH1A1 was tested in a normal colon tissue of mouse. This is a pilot experiment to test the antibody for IF purpose.