DMEM/F12 Medium is a 1:1 mixture of Dulbecco’s Modified Essential Medium (DMEM) and Ham’s F-12 Medium, commonly used in growing a variety of mammalian cultures. DMEM/F12 is rich and complex, containing all 21 amino acids, 10 vitamins, glucose, zinc, and iron among other components. Together, it combines the high amount of glucose, amino acids and vitamins of DMEM, and the diversified components of F-12. It is a synthetic medium containing no serum or proteins and thus may require supplementation depending on the type of cell culture. Some commercial formulations may or may not include glutamine or phenol red.