Terrific Broth

Terrific Broth (TB) is an enriched medium designed for high density bacterial culturing. A one-liter solution typically consists of 12 grams of tryptone, 24 grams of yeast extract, 4 milliliters of glycerol, and potassium phosphate buffer. TB contains slightly more tryptone and more than four times the amount of yeast extract than the standard LB media. Thus TB may be used as an alternative to achieve much greater yields of recombinant E. coli in protein expression or plasmid purification. For convenience, several manufacturers provide ready-made powder and liquid formulations.

Terrific Broth

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