A wide variety of enzymes are available for research applications in cellular and molecular biology. To narrow down your search, select desired filters or enter keywords in the provided text boxes. It may be helpful to note the enzyme source, reaction conditions and units of activity provided by the manufacturers before making a purchase.
Pepsin is a major digestive enzyme produced in the stomach. The native enzyme is most active between 37 °C to 42 °C and at a pH range of 2.0 to 6.5. It uses a catalytic aspartate in the active site and preferentially cleaves connected hydrophobic and aromatic residues. Pepsin can be used in exposing antigens in formalin-fixed tissues to enhance binding in immunostaining and in in situ DNA hybridizations. It is also used in cleaving IgG antibodies to separate the F(ab’)2 fragments from the Fc regions.