Yeast/Fungi DNA Microarray

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Yeast/Fungi DNA Microarray

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Recent Product Review

  • Western Blot of TAP-SPT16 in S. cerevisiae Whole-Cell Lysates

    SPT16 is a component of FACT complex (SPT16-Pob3) in Saccharomyces cerevisiae. FACT complex is involved in the regulation of chromatin-associated processes such as DNA replication and repair, etc. Our aim was to examine the expression of TAP-tagged SPT16 protein in whole-cell lysates of TAP-SPT16 expressing S. cerevisiae cells. We isolated protein from cells by bead beating and loaded on SDS-PAGE and performed western blots using this antibody.

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    Our aim was to study the interaction between H2AZ and other proteins. For that, we purified H2AZ-FLAG protein from S.cerevisiae and examined the levels of H2AZ by using FLAG antibody. The antibody is excellent and gives a specific band corresponding to FLAG.