Anti-TRMT61B antibodies are used in the immunodetection of the protein tRNA methyltransferase 61B. In humans, the canonical protein has a reported length of 477 amino acid residues and a mass of 53 kDa. Its subcellular localization is in the mitochondria. It is notably widely expressed in many tissue types. A member of the TRM61 protein family, TRMT61B is a reported methyltransferase that catalyzes the formation of N(1)-methyladenine at position 58 (m1A58) in various tRNAs in mitochondrion, including tRNA(Leu) (deciphering codons UUA or UUG), tRNA(Lys) and tRNA(Ser) (deciphering codons UCA, UCU, UCG or UCC). Synonyms for this target antigen include mRNA methyladenosine-N(1)-methyltransferase, potential tRNA (adenine(58)-N(1))-methyltransferase catalytic subunit TRMT61B, and tRNA (adenine(58)-N(1))-methyltransferase, mitochondrial. TRMT61B gene orthologs have been reported in the bovine, frog, zebrafish, chimpanzee and chicken species. Some TRMT61B antibodies may have been used in research and have associated citations. These antibodies are most commonly used in Western Blot experiments, among other applications.