Anti-PAM antibodies are used in the immunodetection of the protein peptidylglycine alpha-amidating monooxygenase. In humans, the canonical protein has a reported length of 973 amino acid residues and a mass of 108.3 kDa. Its subcellular localization is in the cytoplasmic vesicles. Up to 6 different isoforms have been reported for this protein. It is notably widely expressed in many tissue types. A member of the Peptidyl-alpha-hydroxyglycine alpha-amidating lyase protein family; Copper type II ascorbate-dependent monooxygenase protein family, PAM is reported to be a bifunctional enzyme that catalyzes the post-translational modification of inactive peptidylglycine precursors to the corresponding bioactive alpha-amidated peptides, a terminal modification in biosynthesis of many neural and endocrine peptides. Synonyms for this target antigen include PHM, pancreatic peptidylglycine alpha-amidating monooxygenase, peptidyl alpha-amidating enzyme, peptidyl-alpha-hydroxyglycine alpha-amidating lyase, and PAL. A number of PAM antibodies have been mentioned in research publications and have associated citations. Western Blot is a widely used application for these antibodies. In addition, ELISA is also a common application.