Anti-MED8 antibodies are used in the immunodetection of the protein mediator complex subunit 8. In humans, the canonical protein has a reported length of 268 amino acid residues and a mass of 29.1 kDa. Its subcellular localization is in the nucleus. Up to 3 different isoforms have been reported for this protein. It is notably widely expressed in many tissue types. A member of the Mediator complex subunit 8 protein family, MED8 is a known component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Synonyms for this target antigen include activator-recruited cofactor 32 kDa component, mediator of RNA polymerase II transcription subunit MED8, and mediator of RNA polymerase II transcription subunit 8. MED8 gene orthologs have been reported in the mouse, frog, zebrafish, chimpanzee and chicken species. Some MED8 antibodies may have been used in research and have associated citations. Western Blot is a widely used application for these antibodies. In addition, ELISA is also a common application.