Site specific endonuclease GlaI recognizes and cleaves only 5mC DNA. This enzyme belongs to a new type of 5mC site-specific DNA endonuclease discovered and characterized at SibEnzyme. The enzyme recognizes DNA sequence 5’-GC↓GC-3’/3’-CG↓CG-5’ with two or more 5-methylcytosines and cleaves it producing blunt ends. GlaI doesn’t cleave unmethylated DNA or DNA with C4- or N6A-modifications. Due to its ability to cleave only cytosine-modified DNA, GlaI may find a practical application in genetic engineering experiments as well as in determination of DNA methylation status in epigenomics studies.
5mC-dependent site-specific DNA endonuclease GlaI
SibEnzyme Ltd. 2/12 Ak.Timakov street, Novosibirsk 630117 RUSSIA Phone: 7-383-333-4991 Fax- 7-383-333-6853 Internet: http://www.sibenzyme.com SibEnzyme US, LLC 2 Franclaire Dr. West Roxbury, MA 02132 Phone: 781-915-7170 Fax:617-553-0856 Internet:http://www.sibenzymeus.com U.S. Customers: http://www.sibenzymeus.com European Customers: http://www.se-technologies.eu Customer Service: Tech Support: Fax Number: Web Site: http://www.sibenzyme.com
View the latest short animated film from BINDER on their KBF series constant climate chambers... watch video
Recently, I investigated the possibility of a novel fusion protein in lung tumors... read more
Stay updated on the latest technologies and news with Biocompare's newsletters (See samples here)