From USB, who introduced Shrimp Alkaline Phosphatase, now comes SuperSAP™, a new formulation designed for quick and convenient DNA dephosphorylation. SuperSAP™ rapidly removes 5' phosphates from protruding, blunt or recessed ends in just 5 minutes at 37°C. It works directly in restriction enzyme digests—just add directly to simultaneously cut and dephosphorylate DNA. No additional buffers or additives are required. SuperSAP™ is 100% heat-inactivated at 65°C. It has all the benefits of SAP with high specific activity and simple heat-inactivation. No purification is necessary prior to ligation.
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