The AmplifluorŪ ID West Nile Virus Reagent is intended for the quantitative or qualitative evaluation of West Nile Virus RNA. This reagent was designed to detect the conserved NS-5 nucleic acid region of the West Nile Virus. Since the West Nile Virus contains an RNA genome, initial amplification reactions are performed using the reverse transcription (RT) primer for 1st strand cDNA synthesis, followed by Polymerase Chain Reaction (PCR) amplification utilizing the forward AmplifluorŪ hairpin primer and reverse primer to produce target-specific fluorescent amplicons. The AmplifluorŪ hairpin primer is labeled with Donor (fluorescein) and Quencher (DABSYL) fluorophores, which produce the high signal to noise ratio observed in the AmplifluorŪ assay. Upon incorporation into new amplicons, the AmplifluorŪ primer is fluorescent (reported in the instruments FAM layer), while the fluorescein signal of the unincorporated AmplifluorŪ primer is quenched.
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