The iFOLD™ Protein Refolding System 1 (Cat. No. 71552) is designed to determine optimal protein refolding conditions by systematically and simultaneously evaluating 92 different buffer combinations of pH, salt, cyclodextrin, redox agent, and refolding additives. The iFOLD system buffer matrix is based on an extensive literature review of successful refolding experiments and information contained in the REFOLD database. The system includes inclusion body purification reagents combined with a dispensed 96-well plate-based protein refolding buffer matrix. Following cell lysis, membrane components and contaminating proteins trapped within inclusion body pellets are removed by a series of detergent and buffer washes. The purified inclusion bodies are denatured by addition of TCEP [Tris(2-carboxyethyl)phosphine] and N-Lauroylsarcosine and refolded by rapid dilution into the iFOLD 96-well buffer matrix. Each kit is sufficient for screening up to 96 refolding conditions (92 experimental and 4 control wells) for a single protein.
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