The P-PER Plant Protein Extraction Kit from Pierce offers a new method for performing plant cell lysis and subsequent protein extraction. The P-PER Kit includes an organic lysing reagent and two aqueous reagents, which in conjunction with mild mechanical agitation, effectively extract plant protein.This gentle extraction procedure avoids harsh mechanical lysis aids, such as a mortar and pestle. Extracts are prepared in just 10 minutes and protein quantitation can be accurately determined with the recently released BCA Protein Assay Kit. The P-PER Kit is an effective tool for extracting protein from a variety of plant tissues. The quantity of protein extracted using the kit is equal to or exceeds conventional extraction methods (i.e., freeze/grinding) and a commercially available plant protein extraction reagent (using equal tissue weight to lysing/ extraction volumes). Additionally, the kit is compatible with 1-D and 2-D gel electrophoresis and other downstream applications, including immunoprecipitation, Western blotting, activity assays and protein purification.
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