USB’s HotStart-IT™ Taq DNA Polymerase uses a novel hot start method designed & developed at USB called primer-sequestration. This new method does not use antibodies or chemically modified enzymes. Instead, a recombinant protein is utilized which binds and sequesters primers at lower temperatures making them unavailable for use by a PCR enzyme. This technology effectively blocks DNA synthesis from mis-priming events at lower temperatures. Following the initial denaturation step during PCR, the protein is inactivated and the primers are free to participate in the amplification reaction. This novel hot start method enhances many complex PCR reactions by increasing both specificity and yield. Also available in a master mix, HotStart-IT™ Taq Master Mix (2X), provides robust and reliable performance.
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