| Biomolecule Search Help |
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« Back to Biomolecule Search
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| Basics |
| The Biomolecule search tool is designed to assist you in finding specific proteins, enzymes, peptides, lipids, carbohydrates, and other biomolecule products. You may search by the name of the biomolecule, the species origin (if applicable), the type of molecule, and/or any conjugate or tag of interest. Using more parameters will narrow your search. |
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| Text Search |
| The default search is by Molecule name. You may narrow your search by choosing the "keyword" radio button and adding more keywords (along with the molecule name) to the text box. Examples of some types of keywords include expression system, CAS number, conjugates or tags, applications, mutations and modifications, etc. |
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| Species Pulldown Menu |
| If you are interested in a biomolecule with a certain species origin, you may narrow your search by selecting one of the options in the pulldown menu. In the case of recombinant proteins and peptides, this selection refers to the origin of the gene sequence, not the expression system. For example, human actin expressed in E. coli would be filed under "human" for species origin. If the species origin is not applicable or not relevant to your search, leave the pulldown menu on the default "Any" value. If your species of interest is not found on the pulldown menu, enter that species name into the text box along with the molecule name for a keyword search. |
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| Type Pulldown Menu |
| This parameter refers to the type of biomolecule you are searching for. Some of the choices overlap; for example a product may be both an enzyme and a protein. Such products are filed under both applicable categories, so they can be found using searches for either category. If you do not know what type your biomolecule falls under, leave the menu on the default "Any" value. If the type you are looking for is not on the pulldown menu, it can be entered into the text box for a keyword search. |
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| Conjugate/Tag Pulldown Menu |
This option allows you to search for biomolecules with a tag or conjugate associated with them. Tags and conjugates are grouped by type as follows:
- Affinity/Antibody binding tags and conjugates are those that are meant to allow purification of the biomolecule or binding with a standard antibody. Examples include GST, biotin, FLAG, agarose, and Histidine tags.
- Fluorogenic tags and conjugates are dyes that either increase in fluorescence or change fluorescent emission wavelengths in a positive experimental result; for example a substrate with a dye and a quencher that is released when the substrate is cleaved. Most FRET pairs fall under this type of conjugate.
- Fluorescent conjugates and tags are dyes that are not meant to change fluorescent characteristics over the course of an experiment. Examples include Rhodamine 110, the Alexa dyes, and GFP.
- Colorimetric conjugates and tags are molecules that will generate a colored product that can be detected visually or quantitated via spectrophotometer.
If you are looking for a specific conjugate molecule, type the name of the conjugate or tag into the text search along with the biomolecule name. You may also choose "Unconjugated" on the pulldown menu if you do not want a tagged or conjugated biomolecule, or leave the menu on the default "Any" if it is not relevant for your search.
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| Additional Tips |
Once you've submitted your search, you will be sent to a results page that lists the number of results you have gotten with your search. If you find that there are too many results, you may narrow the search by adding more keywords to the new search box on the results page. (You'll also notice that your original keyword(s) are still in the text box. Don't remove them or you won't include those terms in your revised search!) You may also further narrow the search by checking specific antibody companies to include from the list provided at the top of the page and clicking "Filter results".
If your search returns too few results, try a new search using fewer parameters.
If this doesn't work, check your spelling and spacing (try a few alternatives with spacing, slashes, or dashes). If your biomolecule has a synonym name, try that, or use a keyword search for a related product in the same pathway or field of study.
« Back to Biomolecules Search
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| If you have further questions or comments, please contact us. Thanks for searching! |
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