| Antibody Search Help |
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« Back to Antibody Search
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| Basics |
| Biocompare's Antibody Search tool has been designed to help you find the right antibodies with minimum effort. You may search by antigen, species reactivity, application, host species, and/or conjugate, as well as by keywords. |
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| Text Search |
| The default setting for the text search is by antigen specificity. To further narrow the search, change the radio button setting to keyword and enter the antigen name plus any keywords you wish to include. Additional keywords include isotype, clonality (poly- or mono-), other conjugates, other species, clone number, vendor name, etc. Using additional keywords narrows your search. |
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| Searches for Secondary Antibodies |
| Type "IgX" or "IgXn" in the antigen field (where "X" or "Xn" is the antibody isotype you'd like to target) and specify the species of origin of the primary antibody you are targeting in the "Reactivity" pulldown menu. (See below for more details). |
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| Searches for Control Antibodies |
| First select the host species from the pulldown menu, then select "keyword" search and enter the isotype ("IgX" or "IgXn") followed by "Isotype Control" (for monoclonals) or "Control Antibody" (for polyclonals). |
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| Searches for Modification Specific Antibodies |
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For phosphorylation state specific antibodies use the following formula: Antigen + phospho + ZZZn where "ZZZ" equals the 3 letter amino acid code (Ser, Thr, or Ser) and "n" equals the residue number (no spaces between the amino acid code and the residue number) - e.g. Histone H3 phospho Ser10.
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For methylation state specific antibodies use the formula above substituting "phospho" with either "methyl", "monomethyl", "dimethyl" or "trimethyl"; and use the 3 letter amino acid codes (Arg, Lys etc) - e.g. Histone H3 dimethyl Lys4.
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For acetylation state specific antibodies use the same formula for phosphorylation state specific antibodies substituting "phospho" with "acetyl" and use the 3 letter amino acid code (Lys etc) - e.g. Histone H2B acetyl Lys85.
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For multiple modifications use the appropriate combination/s - e.g. Histone H3 phospho Ser10 acetyl Lys9, or Histone H3 phospho Thr3 monomethyl Lys4.
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| Reactivity Pulldown Menu |
| This menu refers to the species with which the antibody will react. For example, an antibody may react with human actin but not mouse actin. Use the pulldown menu to select the species you are interested in targeting. If you don't have a particular species in mind, leave the menu on the default "Any". If your species of interest is not on the menu, type it into the text box along with the antigen for a keyword search. |
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| Host Species Pulldown Menu |
| This menu refers to the species that was used to generate the antibody. If this is not important for your search, leave this menu on the default "Any". |
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| Applications Pulldown Menu |
| This refers to which applications the antibody has been qualified or tested for, or its recommended use. If this is not important for your search, leave this menu on the default "Any". If your application is not on the dropdown, type it into the text box along with the antigen for a keyword search. |
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| Conjugate Pulldown Menu |
Many antibodies are conjugated to other molecules for either detection or purification purposes. This pulldown allows you to add the type of conjugate to your search parameters. Fluorescent conjugates are grouped by color (as defined by the emission wavelength). For a table listing the dyes in each color group (and their excitation and emission wavelengths), click here. There is also a link to this table next to the conjugate pulldown menu. To search for a specific conjugate use the dye name and antigen in a keyword search. "Other non-fluorescent conjugates" includes chemiluminescent conjugates, various less common enzymes, affinity matrices (other than agarose/sepharose), I-125, BSA, and colloidal gold, among others.
If you don't need a conjugated antibody, select "Unconjugated" in this menu.
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| Search Results |
Once you've submitted your search, you will be sent to a results page that lists the number of results you have gotten with your search. If there are too many results, you may narrow the search by adding more keywords to the new search box at the bottom of the results page. (You'll also notice that your original keyword(s) are still in the text box. Don't remove them or you won't include those terms in your revised search!) You may also further narrow the search by checking specific antibody companies to include from the list provided at the top of the page and clicking "Filter results".
If your search returns too few results, try a new search using fewer parameters.
If that doesn't work, check your spelling and spacing (try a few alternatives with spacing, slashes, or dashes). If your antigen has a synonym name, try that, or use a keyword search for a related gene product.
« Back to Antibody Search
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| If you have further questions or comments, please contact us. Thanks for searching! |
