Cytotoxicity Colorimetric Assay Kit, PLUS from Roche Applied Science

Description

The Cytotoxicity Detection KitPLUS (LDH) is a fast, sensitive, and simple method to quantitate cytotoxicity/cytolysis based on the measurement of LDH activity released from damaged cells using the 96-well or 384-well plate format. The kit can be used in many different in vitro cell systems when damage to the plasma membrane occurs. For example:

• Detection and quantification of cell-mediated cytotoxicity.
• Determination of mediator-induced cytolysis.
• Determination of the cytotoxic potential of compounds in environmental and medical research, and in the food, cosmetic, and pharmaceutical industries.
• Determination of cell death in bioreactors.

The Cytotoxicity Detection Kit^PLUS (LDH) can be performed in a homogeneous format and requires no transfer and  centrifugation steps to separate the supernatant from the cells. The color reaction can be stopped for defined assay conditions.

Sample material: Cell cultures grown in 96- or 384-well plates can be measured directly. Aliquots from cultures grown in other formats can be transferred into 96- or 384-well plates for measurement without removing the cells.
Assay time: 10 to 30 minutes for incubation.
Sensitivity: Less than 100 lysed cells can be detected in a 96-well plate.

Benefits

• Suitable for high throughput: Fewer handling steps. No transfer, centrifugation, or prelabeling steps are required.
• Flexible: Defined assay conditions when using a stopped color reaction.
• Safe: No radioactive isotopes are used.
• Accurate: Results obtained strongly correlate to lysed cell number.
• Sensitive: Low cell numbers are detected.
• Fast: The use of a multiwell ELISA reader permits a larger number of samples to be processed.