Apo-Direct™ Kit
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Calbiochem
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Product
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Apo-Direct™ Kit
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Company
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Calbiochem
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Price
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Pricing Info
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Catalog Number
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CBA041-1KIT
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Quantity
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50 Tests
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Size
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1 kit
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Applications
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Flow cytometry
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Detection
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Request Info
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Format
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Request Info
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Kit Contents
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A two color TUNEL (Terminal deoxynucleotide transferase dUTPNick End Labeling) assay for labeling DNA breaks and total cellular DNA to detect apoptotic cells by flow cytometry or laser scanning cytometry. This kit utilizes terminal deoxynucleotidyl transferase (TdT) to catalyze incorporation of fluorescein-dUTP at the free 3'-hydroxyl ends of the fragmented DNA.
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Method
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Fluorescence
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Sample Type
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Cells
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Assay Time
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4-5 h
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More Information
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Description
50 Tests. AVOID FREEZE/THAW CYCLES. A two color TUNEL (Terminal deoxynucleotide transferase dUTPNick End Labeling) assay for labeling DNA breaks and total cellular DNA to detect apoptotic cells by flow cytometry or laser scanning cytometry. This kit utilizes terminal deoxynucleotidyl transferase (TdT) to catalyze incorporation of fluorescein-dUTP at the free 3'-hydroxyl ends of the fragmented DNA.
reference:
Lockshin, R.A., 2001.
Nat. Rev. Mol. Cell Biol. 2, 545.Li, X., eta al. 1995.
Cytometry 20, 172.Goldman, A.S., 1983.
Proc. Soc. Exp. Biol. Med. 174, 239.
References
Lockshin, R.A., 2001. Nat. Rev. Mol. Cell Biol. 2, 545.Li, X., eta al. 1995. Cytometry 20, 172.Goldman, A.S., 1983. Proc. Soc. Exp. Biol. Med. 174, 239.