|
|
Cell viability Fluorometric (resorufin) Assay Kit, CytoTox-ONE™ from Promega
| General Product Information |
| Item |
Cell viability Fluorometric (resorufin) Assay Kit, CytoTox-ONE™ |
| Company |
Promega |
| Related Content |
 |
| Price |
$295.00  |
| Catalog Number |
G7891 |
| Quantity |
1,000-4,000 assays |
| Detection |
Request Info |
| Method |
Fluorometric (resorufin) |
| Sample Type |
Cells |
| Category |
Lactate Dehydrogenase (LDH) Cytotoxicity Assays |
| Detection Target |
Cell viability |
| Assay Time |
10 minutes |
| Request Information |
 |
| More Information |
Company Product Page  |
| Product Description |
| The CytoTox-ONE™ Homogeneous Membrane Integrity Assay(a) is a fluorometric method for estimating the number of nonviable cells present in multiwell plates. Cell viability is most often defined based on the integrity of the cell membrane. Measuring leakage of components from the cytoplasm into the surrounding culture medium is widely accepted as a valid method to estimate the number of nonviable cells. The CytoTox-ONE™ Assay rapidly measures the release of lactate dehydrogenase (LDH) from cells with a damaged membrane. LDH released into the culture medium is measured with a 10-minute coupled enzymatic assay that results in the conversion of resazurin into a fluorescent resorufin product. The amount of fluorescence produced is proportional to the number of lysed cells using a 96- or 384-well format. The CytoTox-ONE™ Reagent does not damage normal healthy cells; therefore the reactions to measure released LDH can be performed directly in a homogeneous format in assay wells containing a mixed population of viable and damaged cells. The CytoTox-ONE™ Homogeneous Membrane Integrity Assay, HTP (Cat.# G7892), offers convenient, alternative packaging for processing multiple plates. Each bottle of reagent supplied with the system is sufficient to perform 500 assays in a 96-well format or 2,000 assays in a 384-well format when the recommended volumes are used.
Save Time: Complete the assay in the cell culture plate, eliminating the sample transfer step common in many LDH assays; the plates are incubated for 10 minutes before reading data, compared to 30 minutes or more with classic LDH assays.
Save on Plasticware: Perform in a single multiwell plate without separating cells from supernatant.
Multiplex This Assay: Perform multiple assays on one sample with other homogeneous cell-based assays from Promega.
Adapt Protocol to Your Needs: Treat cells over longer time periods, if desired. Completed assays can be read over several hours after the provided stop solution has been added while still maintaining good signal.
|
NOTE: Biocompare disclaims any information on this site. Price information is approximate list price and actual prices may vary.
|
. Journal of Virology, 79:644-648.
