Human IgG Immunohistochemistry Kit, MaxTag
from
Rockland Immunochemicals, Inc.
Description
This kit relies upon the high specificity of avidin-biotin binding for visualization of an antigen. The antigen (usually within immobilized cells or tissue) is fixed and adhered to a glass microscope slide. A primary antibody reacts with the immobilized antigen to form an antigen-antibody complex. A second biotinylated antibody derived from goat and specific for the human primary antibody reacts with the complex. Streptavidin conjugated to Horseradish Peroxidase reacts with this complex localizing the peroxidase at the site of the antigen. Finally, substrate is added causing a colored precipitate to form on the slide at the location of the antigen. The slide is viewed through a standard light microscope and, if desired, photographed for a permanent record.