The non-viral transfection of genetic material into cells, also known as gene delivery, is simplified by commercially available reagents and kits. The appropriate method for gene delivery depends on the type of cell and genetic material. Plasmid DNA, siRNA, proteins, and antibodies are transfected into cells using methods such as liposomes, calcium phosphate, and electroporation. Proficient stable or transient cell lines are generated using established cell lines, primary cells or stem cells with the appropriate transfection method. In vivo transfection reagents are offered with markers, such as Neomycin resistance. In addition to basic transfection kits, enhancement compounds can be used to increase the efficiency of the selected transfection treatment. A diverse range of transfection products is available to match the required gene delivery method.
PolyJet works very well, especially in HEK293 series cells, such as HEK293, ...
In our experiment, I used Qiagen’s QIAEXII Gel Extraction Kit (Cat. No. 20021) ...
Thursday, January 03, 2013
Transfection, the process of introducing foreign genetic material into a eukaryotic cell, is an important tool...
Transfection is still a major bottleneck for many researchers. Primary cells are valued because they more ...
Thursday, August 26, 2010
The GeneAtlas System is an affordable, easy-to-use microarray solution for life scientists who want a whole-genome view of complex biological questions, but have been held back by budget or the ...
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Wednesday, December 22, 2010
The new Agilent SureSelectXT Automated Library Prep and Capture System streamlines sample preparation of the next-gen sequencing workflow for leading next-generation sequencing platforms — including ...
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This movie guides you through setting up your own successful microarray experiment. You’ll learn about critical steps, and gain all the know-how you need to get started with manual hybridization. ...
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The New AgilePulse® MAX System is the latest addition to the BTX electroporation product line, offering the most advanced solution for large volume ...
The AgilePulse® MAX is a NEW large volume transfection system ideal for fast, efficient transfection of 2 to 6 ml of cell suspension.
Friday, February 08, 2013
Researchers from the Polytechnic University of Catalonia have developed a technique that improves and cuts the cost of a technique called ...