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L-Calc™ Software

L-Calc™ Software

STEMCELL Technologies, Inc.

  • Catalog Number: 28600
  • Description: Limiting Dilution Analysis (LDA) is applied in many areas of research including cell biology, immunology, hematology, microbiology and virology. The aim of such analyses is to estimate the frequency of a cell (within a cell population) capable of a defined responsiveness. For example, limiting dilution analysis has been used to estimate the frequency of lymphocytes which will proliferate or secrete antibodies in response to a specific antigen. In the area of hematology, LDA has been applied to the in vitro quantitation of hematopoietic stem cells using assays such as the long term culture-initiating cell (LTC-IC) assay [E Conneally et al.] and the cobblestone area forming cell (CAFC) assay [J C Wang et al.]. Limiting dilution protocols have also been adapted to allow the quantitation of human and mouse stem cells with the capacity to produce mature cells of all hematopoietic lineages in vivo. These include the competitive repopulating unit (CRU) [R E Ploemacher et al., S J Szilvassy et al.] and the SCID repopulating unit (SRC) assays [H J Sutherland et al.]. L-Calc™ will assist you in analyzing the results of your limiting dilution analysis. For each experiment, values corresponding to dose (i.e. cells per well), total number of positive responses obtained per dose, and total number of replicates tested (i.e. total number of wells used per cell dose) are entered. From these data, the frequency of cells within a given cell population capable of a defined response is determined. L-Calc™ can also be used as a guide to assess differences between paired sets of limiting dilution analyses. A statistician should be consulted to perform the appropriate comparison.
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  • STEMCELL Technologies, Inc.