In the MIDLAND manufacturing and quality control process, the target sequence is also synthesized and the finished molecular beacon probe is tested using the synthetic target. The ratio of signal fluorescence (in presence of target) to background fluorescence (no target present) is measured and reported as analytical data accompanying the product. A copy of the UV/visible absorption spectrum of the purified molecular beacon probe also accompanies the product, as does a sample of the synthetic target sequence.
To meet the increasing market demand, we have introduced the most advanced high-throughput synthesizers, which provide computer-controlled oligo synthesis, cleavage, and deprotection in a parallel-processing format. Our oligos are synthesized with a typical coupling efficiency of 99% and purified by OPC free of charge. PAGE and HPLC purification are also available with additional charge. The high coupling efficiency of our synthesis technology allows efficient synthesis of oligos up to 100 bases. In addition, a variety of modifications are available. Every oligo is analyzed by PAGE to check oligo quality. Malidi-Tof Mass QC is also employed to examine the oligos randomly at our facility. Now we are one of the leading companies in DNA synthesis in China.
Sigma® is recognized as the world’s leading supplier of custom DNA & RNA oligonucleotides for the global life science research community. Our ability to guarantee oligo performance is directly related to our comprehensive understanding of oligonucleotide chemistry and synthesis platforms, our investment in analytical systems and our experience in methods development.
Sigma offers the broadest portfolio of custom DNA primers and probes to support your research. Our product offering includes:
Sigma is committed to providing high quality products to our customers. With an advanced array of state-of-the-art analytical instruments, Sigma routinely analyzes both in-process and final product quality to meet and exceed the high standards of our customers.
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