With strong expertise and the most advanced BIAcore system in hand, Creative Biolabs is capable of satisfying your demands in antibody affinity and kinetics measurement. The optical phenomenon of surface plasmon resonance (SPR) used by Biacore systems enables the detection and measurement of biomolecular interactions in real time and without the use of labels.
At Creative Biolabs, scientists are pleased to use the proprietary Biacore technology and our extensive experience to assess the affinity of your antibodies in various formats (IgG, Fab, scFv and nanobodies). The affinity of the antibody for its antigen is determined by measuring the binding kinetics of the kon, koff; we can also help you define how strongly your antibody binds to its antigen. This is known as the affinity, KD, of the interaction. A high-affinity antibody is typically less than 1 nM.
Antibody affinity is the strength of the reaction between a single antigenic determinant and a single combining site on the antibody. It is the sum of the attractive and repulsive forces operating between the antigenic determinant and the combining site of the antibody. Affinity is also a very important parameter in antibody drug development (i.e.: affinity maturation, humanization).
Besides BIAcore system, scientists from Creative Biolabs also introduced the recently developed ForteBio Octet platform, which is highly applicable in antibody binding affinity measurement and more cost-effective. ForteBio can also be applied in process development, quality analysis, antibody screening, cell line optimization, antigen epitope mapping, antibody pairing, and ligand binding, etc.
Altenatively, you can also choose an ELISA test as a basis for affinity evaluation. We provide classical ELISA and competitive ELISA. For cell-based assay, FACS analysis is frequently used for affinity measurement. Whatever you need, Creative Biolabs can help.