Mammalian Expression Vectors

Mammalian Expression Vectors Specifically engineered mammalian expression vectors produce eukaryotic proteins through transformation or transfection into mammalian cell or in vivo systems. Various techniques are utilized to transcribe DNA from mammalian gene-friendly vectors, like viruses and plasmids, which contain tagged proteins, antibody resistance, and fluorescence reporters. The vector chosen for expression chiefly depends on the gene of interest, but a reporter, like a FLAG tag that will fuse to the expressed protein can also be considered. Fluorescence reporters such as green fluorescent protein (GFP), are activated under specific conditions in localized cell organelles or compartments. The versatile vector system pcDNA 3.1™, from Life Technologies®, is can be used for diverse cloning projects as it adapts to many types of mammalian cells. The vast number of mammalian expression vectors allow for the precise approach for your mammalian system vector cloning and expression.

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