Western blotting, also known as immunoblotting, is a widely-used technique that utilizes antibodies for the detection of a specific protein within a sample. The process involves separating protein samples by electrophoresis and then transferring to a membrane, typically nitrocellulose or PVDF. Detection often involves the use of primary and secondary antibodies where the secondary antibody is conjugated either to an enzyme, such as HRP or AP, or to a fluorescent dye. The target protein can then be visualized using chemiluminescent or chemifluorescent detection reagents, or directly using the fluorescent tag. When purchasing supplies for Western blotting, the type of sample to be analyzed should be taken into consideration. Requirements for a small, relatively pure sample will be very different than those for a crude lysate or other complex mixture.
This product from Lonza is a modified Laemmli SDS-PAGE running buffer that ...
This product from Lonza is a modified Towbin Western blot transfer buffer that ...
Western blotting is the most common method for detecting and quantifying specific proteins. The cost of ...
Monday, February 04, 2013
Though Western blotting has for years been an important mainstay for protein researchers, many believe it has ...
The video gives a brief overview about the simplicity of the workflow realized on the 4D-Nucleofector™:
- Initialization of the system
- Preparation of the transfection samples
- Starting the ... Watch Video
Wednesday, December 22, 2010
The Agilent SurePrint G3 Human CGH+SNP microarrays allow for simultaneous analysis of chromosomal copy number changes and copy-neutral aberrations like loss of heterozygosity (LOH) and uniparental ... Watch Video
Molecular Surgery is a technique that can be used in any laboratory to
specifically eliminate cells. Find out how to use targeted toxins to
advance your research. Watch Video
The Combipack™ is Porvair Sciences economic response to the growing use of protein precipitation separation techniques in chromatography labs.
ORLANDO, Fla., August 13, 2012 – To survive, tumors need blood supply to provide them with nutrients and oxygen. To get that supply, cancer cells ...
Thursday, February 16, 2012
Porvair Sciences has announced a new range of high performance 96- well ELISA microplates for diagnostic and immunological research applications.