Western blotting, also known as immunoblotting, is a widely-used technique that utilizes antibodies for the detection of a specific protein within a sample. The process involves separating protein samples by electrophoresis and then transferring to a membrane, typically nitrocellulose or PVDF. Detection often involves the use of primary and secondary antibodies where the secondary antibody is conjugated either to an enzyme, such as HRP or AP, or to a fluorescent dye. The target protein can then be visualized using chemiluminescent or chemifluorescent detection reagents, or directly using the fluorescent tag. When purchasing supplies for Western blotting, the type of sample to be analyzed should be taken into consideration. Requirements for a small, relatively pure sample will be very different than those for a crude lysate or other complex mixture.
In our early studies, we sought to catalog the humoral immune response in dozens...
This product from Thermo Scientific (Pierce) is a cost effective ...
Tuesday, December 03, 2013
Gel documentation systems automate the process of gel photography. Once the image is captured, additional ...
Thursday, October 10, 2013
Many researchers depend on the effectiveness and specificity of commercially available, phosphospecific ...
Tuesday, November 19, 2013
GE Healthcare Life Sciences introduces ÄKTATM Start, automated protein purification. Protein purification is easy with prepacked columns, built in protocols, and an integrated touchscreen. Watch Video
In this video we will share with you tips and tricks for getting the best out of HisTrap(tm) FF Crude column. HisTrap FF Crude column allows you to purify histidine-tagged proteins directly from ... Watch Video
Wednesday, February 17, 2010
This presentation will describe the use of Stem Cell-Derived Human Cardiomyocytes (SC-hCMs) in action potential assays. Data will be presented highlighting the distinct pharmacological sensitivity and... Watch Video