Western Blotting

Western Blotting Western blotting, also known as immunoblotting, is a widely-used technique that utilizes antibodies for the detection of a specific protein within a sample. The process involves separating protein samples by electrophoresis and then transferring to a membrane, typically nitrocellulose or PVDF. Detection often involves the use of primary and secondary antibodies where the secondary antibody is conjugated either to an enzyme, such as HRP or AP, or to a fluorescent dye. The target protein can then be visualized using chemiluminescent or chemifluorescent detection reagents, or directly using the fluorescent tag. When purchasing supplies for Western blotting, the type of sample to be analyzed should be taken into consideration. Requirements for a small, relatively pure sample will be very different than those for a crude lysate or other complex mixture.

Western Blotting

  • DIY Antibodies for Western Blotting

    DIY Antibodies for Western Blotting
    Tuesday, March 04, 2014
    The key to a good Western blot is the antibody, yet sometimes, the right antibody simply isn't available. In ...
  • Gel Documentation Software

    Squeeze Data from Agarose with these Gel-Analysis Software Tools
    Tuesday, December 03, 2013
    Gel documentation systems automate the process of gel photography. Once the image is captured, additional ...