Western Blotting

Western Blotting Western blotting, also known as immunoblotting, is a widely-used technique that utilizes antibodies for the detection of a specific protein within a sample. The process involves separating protein samples by electrophoresis and then transferring to a membrane, typically nitrocellulose or PVDF. Detection often involves the use of primary and secondary antibodies where the secondary antibody is conjugated either to an enzyme, such as HRP or AP, or to a fluorescent dye. The target protein can then be visualized using chemiluminescent or chemifluorescent detection reagents, or directly using the fluorescent tag. When purchasing supplies for Western blotting, the type of sample to be analyzed should be taken into consideration. Requirements for a small, relatively pure sample will be very different than those for a crude lysate or other complex mixture.

Western Blotting

  • Western Blot Documentation

    Western Blot Documentation Systems: Buyers Guide 2014
    Tuesday, July 22, 2014
    Given the many options, selecting a system for protein and Western blot documentation can be overwhelming. The...
  • Protein Quantitation with SRM

    Let Your Westerns Ride Off Into the Sunset, Multiplex Protein Quant with SRM Instead
    Tuesday, June 24, 2014
    For higher levels of multiplexing, researchers in the proteomics community increasingly are keeping their ...
  • C-DiGit Chemiluminescent Western Blot Scanner

    C-DiGit Chemiluminescent Western Blot Scanner
    The C-DiGit Blot Scanner is a truly affordable digital replacement for film that combines the sensitivity of film with the convenience and ...
  • Epitope Tag Antibodies

    Epitope Tag Antibodies
    Epitope-tagged antibodies recognize a specific sequence of amino acids of a recombinant protein. These antibodies are optimal for ...