Phosphatase Substrates (Fluorescent)

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FDP [Fluorescein diphosphate, tetraammonium salt]

AMSBIO LLC

Catalog Number: 11600-5mg
Description: FDP [Fluorescein diphosphate, tetraammonium salt]

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Fluorescein diphosphate, tetraammonium salt (FDP)

AnaSpec, EGT Group

Catalog Number: 85300
Description: Fluorescein diphosphate (FDP) is one of the most sensitive fluorogenic phosphatase substrates commercially available. The colorless and nonfluorescent FDP is hydrolyzed to highly fluorescent fluorescein, which exhibits excellent spectral properties that match the optimal detection window of most fluorescence instruments. Alternatively, FDP can also be used to detect phosphatases in a chromogenic mode since the enzymatic product (fluorescein) exhibits a large extinction coefficient (close to 100,000 cm-1mol-1). FDP has been widely used in various ELISA assays. Additionally it is also used to detect tyrosine phosphatases. FDP is not very thermally stable, and special cautions need be excised for storing the solid sample and stock solutions.

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ECF Substrate

GE Healthcare, formerly Amersham Biosciences

Catalog Number: RPN5785
Description
: ECF™ Substrate for Western Blotting
- A fluorescent substrate for alkaline phosphatase-based detection of protein blots, for use with fluorescence scanning equipment such as Storm™ instruments.
- For use in Western blotting, dot, and slot blotting applications.
- Sensitivity comparable to chemiluminescence.
- The wider dynamic range of fluorescence scanning systems compared with film allows visualization and quantitation of weak and dark bands in the same scan.
- Eliminates need for darkroom and film.

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HNPP Fluorescent Detection Set; For sensitive fluorescent detection of nonradioactively labeled nucleic acids in fluorescence in situ hybridization (FISH) and membrane hybridization

Roche Applied Science

Catalog Number: 11758888001
Description: Evaluation: fluorimetric; absorbance max. 290 nm, emission max. 509 nm
Formula: HNPP: C₂₃H₁₈NO₅P, Fast Red TR: C₇H₆Cl₂N₂ x 1/2 ZnCl₂
M

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4-Methylumbelliferyl phosphate, disodium salt trihydrate
Santa Cruz Biotechnology, Inc.

Catalog Number: sc-206916
Description: 4-Methylumbelliferyl phosphate, disodium salt trihydrate

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O-Methyl-O-(N-Butylfluorescein)phosphate
Santa Cruz Biotechnology, Inc.

Catalog Number: sc-219445
Description: O-Methyl-O-(N-Butylfluorescein)phosphate

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