Confocal microscopy is a fluorescence-imaging technique that produces exquisitely sharp optical sections through biological specimens such as tissue slices. The technique generally relies upon rasterizing an excitation laser over the sample and collecting emission data, point by point, to reconstruct the final image. There are two fundamental ways to build a confocal microscope. Laser-scanning microscopes rasterize the excitation laser across the sample, building an image point by point. The emitted light from any given point (or pixel) typically is captured by one or more point detectors, such as photomultiplier tubes (PMTs), then fed to a computer, which then reassembles the image... read more
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