StarGate® Newcomer Set
from
IBA GmbH
Description
StarGate Newcomer Set consists of the StarGate Entry Cloning Set and the StarGate Transfer Reagent Set.
The two sets allow for getting started with StarGate: Use the Stargate Entry Cloning Set to create up to 20 different Donor Vectors containing your genes of interest. Thereafter, use the StarGate Transfer Reagent Set to transfer each gene of interest from the Donor Vector directly into the selected Acceptor Vector(s). Required Acceptor Vectors have to be ordered additionally according to your needs.
StarGate® is a cloning system suitable for the transfer of any gene of interest systematically into E. coli, yeast, mammalia and other expression systems. StarGate allows the parallel combination of a gene of interest (GOI) with different promoters (i.e. hosts), purification tag sequences or other genetic elements in a one-tube reaction. The core element of this new technology is the site-specific combinatorial enzyme formulation StarCombinase®, that makes cloning versatile, fast, easy and safe.
Unique features are the short combinatorial sites (≤ 2 amino acids) not disturbing the gene of interest, the high level cloning efficiency, the availability of a multitude of combinatorial sites allowing e.g. efficient fusion cloning, and the competitive pricing structure.
.StarGate Entry Reagent Set (20rxns)
StarGate Transfer Reagent Set (20rxns)
For/ Rev Sequencing Primer
DNA Ruler
competent E. coli Top10 cells (20 rxns)
Control for Entry and Transfer reaction
Specifications/Features
Entry Vector pENTRY-IBA
StarSolution M1 , M2 , M3
competent E. coli Top10 cells
For/ Rev Sequencing Primer
DNA Ruler
Acceptor Vectors (20 rxn, max. 4 different vectors of choice, 5 rxn each)
StarSolution A1, A2, A3 for 20 rxn
competent E. coli Top10 cells (20 rxn)
Advantages:
- Minimal modification of the gene of interest due to short combinatorial sites (4 bases only)
- Easy-to-handle subcloning procedure
- Systematic screening of different elements (e.g. tags/promoters) in a variety of hosts
- Inherent high level cloning efficiency due to a directed reaction (no equilibrium)
- Availability of a multitude of combinatorial sites for combinatorial cloning