SulfoLink Immobilization Kit for Proteins
from
Thermo Scientific Pierce Protein Research Products
Description
SulfoLink Coupling Resin is porous 6% crosslinked beaded agarose that has been activated with iodoacetyl groups. When incubated with a solution of peptide or protein that contains reduced cysteine residues, the iodoacetyl groups react specifically and efficiently with the exposed sulfhydryls (-SH) to form covalent and irreversible thioether bonds that permanently attach the peptide or protein to the resin. The result is a custom-made affinity resin for purification of antibodies, antigens and other molecules of interest.
Sufficient reagents to prepare five affinity columns.
Kit contains:
SulfoLink Columns 5 x 2 ml
SulfoLink Sample Preparation Buffer 7.5 ml
SulfoLink Coupling Buffer 500 ml
Wash Solution 120 ml
2-Mercaptoethylamine•HCl 5 x 6 mg
L-Cysteine•HCl 100 mg
Zeba Desalt Spin Columns 5 x 5 ml
Phosphate Buffered Saline Pack 1
Columns Accessories