Klenow Fragment (3'-5' exo-)
from
New England Biolabs
Description
- Isolated from a recombinant source
- Generates probes using random primers
- Specific Activity: 20,000 units/mg
- Dideoxy sequencing
- Supplied with 10X Reaction Buffer
- Moderate strand displacement activity
Description:
Klenow Fragment (3´→ 5´ exo- ) is an N-terminal truncation of DNA Polymerase I which retains polymerase activity, but has lost the 5´→ 3´ exonuclease activity and has a mutation (D355A, E357A) which abolishes the 3´→ 5´ exonuclease activity .
Source:
An
E. coli strain containing a plasmid with a fragment of the
E.coli polA (D355A, E357A) gene starting at codon 324.
Applications:
- Random priming labeling
- DNA sequencing by the Sanger dideoxy method
- Second strand cDNA synthesis
- Second strand synthesis in mutagenesis protocols.
Reagents Supplied:
>NEBuffer 2
Specifications/Features
Unit Definition:
One unit is defined as the amount of enzyme required to convert 10 nmol of dNTPs to an acid-insoluble material in 30 minutes at 37°C.