HisPur Cobalt Spin Columns, 1 ml
from
Thermo Scientific Pierce Protein Research Products
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Product
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HisPur Cobalt Spin Columns, 1 ml
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Company
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Thermo Scientific Pierce Protein Research Products
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Price
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Pricing Info
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More Information
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View Company Product Page
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Capacity
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greater or equal to 10 mg at greater than 90% purity of a 28 kDa His-tagged protein from a bacterial source per milliliter of settled resin
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Matrix
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HisPur IMAC Resin crosslinked 6% agarose in a 20% ethanol solution
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Quantity
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5 columns
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Catalog Number
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89968
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Description
HisPur Cobalt Resin enables efficient purification of polyhistidine-tagged proteins from bacterial, mammalian and baculovirus-infected cells. His-tagged proteins are purified from total soluble protein extract using a cobalt-charged tetradentate chelator immobilized onto 6% crosslinked agarose.
The cobalt resin is compatible using native or denaturing conditions and can be used in multiple formats including conventional gravity flow chromatography, spin column and FPLC. Many immobilized metal affinity chromatography (IMAC) resins use nickel (Ni+2) as the metal source for purifying His-tagged proteins. Although Ni+2 chelate resins achieve high protein yields, purity is often suboptimal, resulting in the need for additional optimization of wash and elution steps. Cobalt achieves both high protein yield and purity with minimal optimization. Furthermore, HisPur Cobalt Resin displays less metal leaching compared with Ni+2 resins.