Description
ACAT2 (acetyl-Coenzyme A acetyltransferase 2 (acetoacetyl Coenzyme A thiolase)) Antibody
Description: Acetyl-Coenzyme A acetyltransferase 2 is an enzyme involved in lipid metabolism. Reported patients with ACAT2 deficiency have shown severe mental retardation and hypotonus. The ACAT2 gene shows complementary overlapping with the 3-prime region of the TCP1 gene in both mouse and human. These genes are encoded on opposite strands of DNA, as well as in opposite transcriptional orientation.
Accession Number: NP_005882
Nucleotide Accession Number: NM_005891
Swiss Protein ID: Q16146
Peptide ID: P03810
Antigen Specificity: Polyclonal antibody produced in rabbits immunized with a synthetic peptide from human ACAT2 with an internal ID of P03810
Protein Size: 397
Molecular Weight: 44kDa
Reconstitution & Storage: Antibody is lyophilized from PBS buffer with 2% sucrose. Add 100 µl of distilled water. Final antibody concentration is 1 mg/ml. For longer periods of storage, store at -20°C. Avoid repeat freeze-thaw cycles.
Applications:
Western Blot: Suggested dilution at 1.0µg/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody.
ELISA titre using peptide based assay: 1:12500
References
Sakashita,N.,et al., (2003) Lab.Invest.83(11),1569-1581