T4 Polynucleotide Kinase (3' phosphatase minus)
from
New England Biolabs
Description
T4 Polynucleotide Kinase catalyzes the transfer and exchange of Pi from the ? position of ATP to the 5´ -hydroxyl terminus of double- and single-stranded DNA and RNA, as well as nucleoside 3´-monophosphates (1-5). This modified version exhibits full kinase activity with no 3´ phosphatase activity.
Specifications/Features
Unit Definition:
One unit is defined as the amount of enzyme catalyzing the incorporation of 1 nmol of acid-insoluble [32P] in 30 minutes at 37°C