DNA Methylation Sequencing
from
ACGT, Inc.
Description
We offer a full service analysis to determine the location and extent of cytosine methylation patterns in the DNA. Genomic DNA is purified and reacted with a bisulfite reagent that converts cytosine to uracil, but leaves 5-methylcytosine intact. A target region from unreacted- and bisulfite-treated DNA is PCR amplified. During amplification, uracil is treated as a thyimidine and 5-methylcytosine as a cytosine. DNA sequencing of the PCR products from the unreacted and bisulfite-treated DNA reveals the location of methylated cytosines.
When the methylation pattern of a DNA fragment is compared across treatments, (i.e., age, exposure to mutagenic chemicals, metastasis) this analysis is able to pinpoint putative epigenetic elements in the DNA. Variations of our services include high throughput screening of new and previously defined methylation-sensitive DNA regions across treatment conditions.
Specifications/Features
Features:
- DNA extraction
- PCR and sequencing of the target sequence
- Bisulphite conversion of unmethylated cytosines
- Optimized PCR amplification of bisulphite converted target DNA
- PCR sequencing and sequence comparison to the given or generated reference