SBF-F
from
AnaSpec
Description
SBF-F is a water-soluble reagent that reacts with thiol groups to produce highly fluorescent products. The SBF-thiol adduct has absorption maximum at 385 nm and fluorescence maximum at 515 nm. It is widely used for HPLC derivatizations of thiol compounds. The HPLC detection limits of thiol compounds such as glutathione, cysteine, N-acetylcysteine, CoA and BSA are in the range of 100-500 pmol/injection. Unlike SBF-Cl, it also reacts with amine compounds in aqueous solutions.
References
Uchiyama S, et al. (2001). Fluorogenic and fluorescent labeling reagents with a benzofurazan skeleton. Biomed Chromatogr 15, 295-318; Fermo I and Paroni R (2000). Total plasma homocysteine analysis by HPLC with SBD-F precolumn derivatization. Methods Mol Biol 159, 237-44.
Specifications/Features
Storage:
refrigerated and desiccated