pDUO2-mcs
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InvivoGen
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Product
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pDUO2-mcs
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Company
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InvivoGen
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Price
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Pricing Info
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More Information
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View Company Product Page
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Host
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E. coli, Mammalian
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Type
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Plasmid
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Applications
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Expression, Cloning, Other
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Catalog Number
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pduo2-mcs
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MCS
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MCS1 includes the following restriction sites:
Age I, Eco RV, Bam HI, Sal I and Avr II - Age I is compatible with Bsp EI and Sgr AI.
- Eco RV is compatible with any blunt-end restriction
- Bam HI is compatible with Bgl II, Bst YI and Bcl I.
- Sal I is compatible with Ava I and Xho I.
- Avr II is compatible with Xba I, Spe I and Nhe I. MCS2 includes the following restriction sites:
Sgr AI, Bgl II, Xho I and Nhe I - Sgr AI is compatible with Bsp EI and Age I.
- Bgl II is compatible with Bam HI, Bst YI and Bcl I.
- Xho I is compatible with Ava I and Sal I.
- Nhe I is compatible with Xba I, Spe I and Avr II.
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Origin of Replication
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pMB1 Ori: a minimal E. coli origin of replication to limit vector size, but with the same activity as the longer Ori.
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Promoter
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• EM7 :
EM7 is a bacterial promoter that enables the constitutive expression of the antibiotic resistance gene in E. coli. • hFerH and hFerL composite promoters:
Ferritin is a 24 subunit protein composed of two subunit types, termed H (heavy) and L (light), which perform complementary functions in the protein. Ferritin is ubiquitously expressed. Its synthesis is highly regulated by the iron status of the cell. The iron regulation is achieved at the translational level through the interaction between the ironresponsive element (IRE), located in the 5’ untranslated region (5’UTR) of the ferritin mRNAs, and the iron regulatory protein4. To eliminate the iron regulation of the ferritin promoters, the 5’UTR of FerH and FerL have been replaced by the 5’UTR of the mouse and chimpanzee elongation factor 1 (EF1) genes, respectively.
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Quantity
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Lyophilized transformed E. coli
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Resistance
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Hygromycin Hph (hygromycin resistance gene): confers resistance to Hygromycin B both in E. coli and mammalin cells. In bacteria, hph is expressed from the constitutive E. coli EM7 promoter. In mammalian cells, hph is transcribed from the human FerH composite promoter as a polycistronic mRNA and translated via the FMDV IRES
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Selectable Marker
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Blasticidin
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Vector Name
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pDUO2-mcs
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Description
Plasmid with two multiple cloning sites. Negative control for pDUO plasmids. Selectable with hygromycin B in mammalian cells and in E. coli.
Specifications/Features
Kit Contents:
* Each pDUO plasmid is provided as a lyophilized transformed E. coli strain on a paper disk.
* Transformed strains are shipped at room temperature and should be stored at -20°C.
* Lyophilized E. coli cells are stable for at least 1 year when properly stored.
* Each pDUO plasmid is provided with 4 pouches of the appropriate E. coli Fast-Media© (2 TB and 2 Agar).
Features:
• SV40 enhancer which is comprised of a 72-base-pair repeat allows the enhancement of gene expression in a large host range. The enhancement varies from 2-fold in non-permissive cells to 20- fold in permissive cells. Furthermore, the SV40 enhancer is able to direct nuclear localization of plasmids.
• CMV enhancer: The major immediate early enhancer of the human cytomegalovirus (HCMV), located between nucleotides -118 and -524, is composed of unique and repeated sequence motifs. The HCMV enhancer can substitute for the 72-bp repeats of SV40 and is severalfold more active than the SV40 enhancer.
• SV40 pAn: the Simian Virus 40 late polyadenylation signal enables efficient cleavage and polyadenylation reactions resulting in high levels of steady-state mRNA. The efficiency of this signal was first described by Carswell et al.
• FMDV IRES: The internal ribosome entry site of the Foot and Mouth Disease Virus enables the translation of two open reading frames from one mRNA with high levels of expression.
Vector Size:
6321 bp