siPORT™ Amine Transfection Agent
from
Ambion
Description
A polyamine-based Ambion® siRNA transfection agent that can be used to transfect cells during subculturing-—saving a full day of valuable time—without increasing toxicity. It is supplied in one tube containing 0.4 mL.
A successful siRNA experiment requires the appropriate transfection agent for your cells, and one that is optimized for siRNA delivery. The efficiency with which different transfection reagents deliver nucleic acids can vary dramatically with the type of nucleic acid being transfected (RNA vs. DNA, single-stranded vs. double-stranded) and the cells being used.
Accessory Products:
siPORT™ NeoFX™ Transfection Agent (SKU#s AM4511 and AM4510), a cationic and neutral lipid mixture, is also available. For testing which transfection agent is best with your system, the Silencer® siRNA Transfection II Kit (SKU# AM1631) is recommended. It contains the two different transfection agents along with controls for transfection optimization.
Specifications/Features
Advantages:
Ambion has developed transfection agents optimized for the transfection of siRNA into a wide variety of cultured cells: siPORT™ Amine (a polyamine mixture) and siPORT™ Lipid (a mixture of cationic and neutral lipids). As expected, the two agents have different transfection efficiencies for different cell types (summarized in the chart, "Recommended siPORT Transfection Reagent for High Efficiency Transfection", at left under Supplementary Protocols), but together allow siRNA to be introduced into a broad variety of cultured cell types.